minirhizotron camera systems

key instruments enabling to study of root and rhizosphere processes



overview

ESVSI-BARTZ Minirhizotron camera systems and Accessories are key instruments enabling to study root and rhizosphere processes and the related ecosystem and biogeochemical processes.

Minirhizotron Imaging Technique

Observing the development of root systems is key to understand plant performance in natural and production ecosystems (trees and crops), and i.a. an important mean to unravel water, carbon and nutrient dynamics of terrestrial ecosystems. Non-destructive images of roots and the rhizosphere can be used to determine a variety of parameters (under controlled or abiotic and biotic stress conditions) including root system development, observation of growth and turnover patterns, root distribution per depth (root system architecture, RSA), occurrence of mycorrhizal root tips and hyphae development, rhizobia development and soil fauna observations. However, observing this "hidden half" of ecosystems is not easy, being covered in the soil. Minirhizotron (MR) systems are a qualitative and quantitative observation tool to study root and hyphal growth, longevity and distribution in situ or mesocosms experiments.

MR systems are based on transparent tubes (MR-T) inserted tightly into the soil. Subsequently an minirhizotron imaging device is inserted into the tube in order to record images of roots and rhizosphere seen through the MR tube walls. The images are then recorded and processed by a control unit.

ESVSI MS

ESVSI MS-190S MINIRHIZO Starter Set. The complete set includes everything needed to install and run an replicated minirhizotron experiment. It is especially suitable for users who want to start using the minirhizotron technology to study root system development and root turnover.

features

Ready to use set, including manual VSI MS-16 camera set, 20 MR tubes and a fitting soil corer to install the tubes manually.

  • Ultra-high resolution (2500 dpi)
  • "Manageable" image size (max. 34 mm x 24 mm in 7 cm MR tube) for manual root tracing
  • Very fast imaging (<1 s), for efficient imaging campains
  • Live root images, important for any screeing purposes
  • UI Option: image resizing (20 mm x 20 mm) and correction of non-linearity (due to MR tube curvature)
  • Accurate and robust indexing system (Smucker handle), Optional: with novel indexing head for fast, reliable circular positioning
  • 12V (3A) system, fully field, and greenhouse operable; Optional: internal, rechargeable batteries
  • Usable in horizontal, vertical  and angles MR tubes; MR tube dimensions compatible to BARTZ and MR scanner systems; image MR tubes of up to 500 cm in lenght

ESVSI MR

Complete starter pack, including MR camera ESVSI MS-190, 20x MR tubes and coring set for MR tube installation.

features

Camera features

  • Ultra-high resolution (2500 dpi)
  • "Manageable" image size (max. 34 mm x 24 mm in 7 cm MR tube) for manual root tracing
  • Very fast imaging (<1 s), no "white calibration" necessary, for efficient imaging campaigns
  • Live root images, important for any screening purposes
  • NEW user-friendly ICAP Software on Windows OS
  • ICAP naming scheme allows image analysis in a range of free and commercial software applications
  • Accurate and robust indexing system (classical "Smucker" handle with novel indexing head for fast, reliable circular positioning) - NOW smaller
  • Battery operated system (>12 h), fully field and greenhouse operable
  • Usable in horizontal, vertical and angled MR tubes

MR tubes

  • 7 cm Acrylic tubes compatible to MR scanner systems, 1 m long, with 2 end caps each (top removable)

Coring system

  • Manual coring set, 68 mm outer diameter to tightly install tubes (max. soil depth ca. 90 cm if installed vertically)

ESMS-190

ESVSI-BARTZ MS-190, Manual UHD Minirhizotron Camera. The instrument allows wide range of basic or applied plant and soil scientists to capture UHD digital images (up to 2500 dpi!) of roots and the rhizosphere over one or multiple growing seasons. Durable and lightweight, and equipped with the reliable "Smucker" indexing handle for exact vertical positioning (and an novel indexing head for circular positioning), the manual & portable UHD minirhizotron camera system is following the tradition of Bartz camera systems. It is reliable and easy to transport to any field location or can be used in rhizotron facilities and for studies in mesocosms. It is equally suitable to image horizontally, vertically and angled minirhizotron tubes up to 500 cm in length and can be factory calibrated to a large range of different MR tube dimensions. The Windows OS-based ICAP 8 software quickly and reliable allows to set-up and follow-up imaging schedules and locations and handles the comprehensible ICAP naming of images.

features
  • Ultra-high resolution (2500 dpi)
  • "Manageable" image size (max. ca. 34 mm x 24 mm in 7 cm MR tube) for manual root tracing
  • Very fast imaging (<1 s), no "white calibration" necessary, for efficient imaging campaigns
  • NEW ICAP UI: user-friendly, Windows-based ICAP software
  • Live root images, important for any screening purposes
  • Experiment manager to set-up and follow your imaging progress
  • Regulate resolution, lighting etc.
  • Accurate and robust indexing system (classical "Smucker" handle with novel indexing head for fast, reliable circular positioning) - NOW smaller in size
  • Battery-powered system (Laptop AND internal power bank), fully field and greenhouse operable for >12 h (@Temp 10-30°C) + external charging option
  • Usable in horizontal, vertical and angled MR tubes; MR tube dimensions compatible to BARTZ and MR scanner systems; MR tubes of up to 500 cm in length!

ESMS-300

ESVSI MS-300, Semi-automatic minirhizotron camera system. The system is designed to be operated in horizontally or flat-angled installed MR tubes as commonly installed in rhizolabs and other experimental installations holding large amounts of minirhizotron tubes. The dual view system (i.e. 2 cameras on opposite sides of the imaging unit) is wirelessly programmed with the MR tube number via a table computer and placed on a gear rack slightly protruding from the MR tubes. After initialization, the camera module is independently and precisely taking photos at pre-set positions (via handheld computer) before returning to the home position. This allows an operator to run several camera modules efficiently in parallel - being only tasked with changing camera modules between tubes and setting the MR tube numbers via scanning RFID tags with the handheld. Images are saved in ultra-high resolution (2500 dpi) and with the ICAP naming scheme on one of two USB sticks (16 GB) per camera module and can thus be transferred anytime to a laptop etc. The battery of the camera module is charge and replaceable for virtually unlimited time of operation. The software allows to pre-set imaging positions in bulk or individually per tube, taking e.g. differently protruding MR tubes into account. The circular gear rack position can be manually changed with magnetic end caps (indexing heads) at the end of tubes (to image the complete inside of the MR tube). Gear racks can either remain in tubes (most convenient) or can be interchanged between tubes (to save set-up costs).

features
  • Automatic imaging after insertion in tube/gear rack (all or pre-set positions)
  • Dual cameras (opposite field of view) with ultra-high resolution (2500 dpi)
  • "Manageable" image size for manual root tracing
  • Very fast imaging (<1 s) of two sites, no "white calibration" necessary, for efficient imaging campaigns
  • UI Option: image resizing and correction of non-linearity (due to MR tube curvature)
  • Accurate depth positioning on gear rack, circular positions manually changeable
  • Lithium-ion battery operated, batteries user changeable
  • Designed for horizontal and slightly angled MR tubes; MR tube dimension compatible to MR scanner systems; MR tubes of up to 200 cm in length!
  • Programming of imaging units with tablet computer and tube # recognition via RFID / Bluetooth
  • One operator can run several imaging heads in parallel for large MR facilities

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